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Pregnyl se daje kao supstitucija za endogenu sekreciju LH, u sredini ciklusa, da bi se postigla finalna faza sazrevanja folikula, što je neophodno za ovulaciju. Pregnyl se, takođe, daje kao supstitucija za endogeni LH u lutealnoj fazi.Kod infertilnih žena koje su u programu asistirane reprodukcije, naročito kod IVF-a, često postoje i abnormaln